PCR LABORATORY
The Biotechnological division of Chelab is composed by a highly-qualified and specialized staff of six bachelors in Biological Sciences, in Natural Sciences, in Biotechnologies and Pharmaceutical Sciences.
PCR surveys are distributed in five separated rooms, dedicated to the operation of sample grinding, extraction of DNA, mix reaction preparation, enlargement and surveying of DNA: the aim is to avoid the cross contaminations. All the analytical methods used are validated by UNI CEI EN ISO/IEC 17025:2005 (SINAL) and/or conducted in accordance with Good Practise of Laboratory (GPL).
The laboratory offers to its costumer qualitative and quantitative PCR survey services (Polymerase Chain Reaction) for the detection of Genetically Modified Organisms (GMO) (in particular soy bean and maize) on food destined to human and zootechnical use and for the quantification of the presence of soft grain in pasta of hard grain; analysis in Multiplex PCR and RFLP for the identification of animal species in foodstuffs (distinction between bovine, ovine, caprine, swine, buffalo, avicultural); screening through PCR Real Time of allergens.
The continuous up-dating of the analysts, supported by the numerous collaborations with research centre and university and the knowledge of PCR’s techniques allowed to reach significant aims in the application of other research methods based on DNA. The service of bacteria characterization is already active through gene sequencing for the 16S RNA 500 or 1500 bases and the traceability of beef meat through SSR.
The application of the DNA-Finger Printing techniques (e.g., RFLP, RAPD, AFLP) is useful to the individuation of polymorphisms for the creation of genic maps, the reconstruction of the origins of different biological materials (animal species and vegetable varieties) and the study of the population biology.
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Furthermore, the division is already active in the analytical control of the bactericidal and fungicidal activity in GLP (with all the references for the registration of medical devices) of chemical disinfectants and antiseptic products used in different sectors, in industries, at home, in hospital according or in conformity with many international methods such as:
- UNI EN 1276:1997 bactericidal activity with interferents
- UNI EN 1650:2000 fungicidal activity with interferents
- EN 13704:2002 spore activity in suspension
- UNI EN 13697:2001 surface microbicide activity
- UNI EN 1500:1997 bactericidal activity of hand disinfectants on volunteers
The laboratory is equipped with:
- ABI PRISM® 7700 SDS detection with ionic Argon laser detector for quantitative PCR and PE 9700 thermocycler
- ABI PRISM® 3100 Genetic Analyzer: sequencer with 16 capillaries
- ABI PRISM® 3130 XL Genetic Analyzer: sequencer with 4 capillaries
- Beckman/Coulter EPICS XL-MCL cytofluorimeter
- Devices for cell cultures
- GeneAmp 9700 thermocyclers
- Electrophoresis systems